FIG. 3.

Effects of insulin and glucagon on ADD1/SREBP-1c gene transcription in cultured hepatocytes. (A) Hepatocytes were cultured for 16 h in the presence of 5 mM glucose (G 5) and then incubated for 1 h with 5 mM glucose in the presence or absence of 100 nM insulin (Ins). (B) Hepatocytes were cultured for 16 h in the presence of 5 mM glucose and 100 nM insulin and then incubated for 1 h in the presence of 5 mM glucose and 100 nM insulin with or without 1 μM glucagon. Nuclei were isolated from cultured cells as described in Materials and Methods. The radiolabeled transcripts were hybridized to dot-blotted cDNAs for ADD1/SREBP-1c, PEPCK, and albumin and to pSV Sport1, the plasmid into which ADD1/SREBP-1c cDNA was cloned. The blot is representative of two experiments.