FIG. 4.

Effects of insulin and dibutyryl-cAMP on ADD1/SREBP-1c gene expression in the presence of cycloheximide. (A) Hepatocytes were cultured for 16 h in the presence of 5 mM glucose and then incubated 15 min in the presence of 5 μM cycloheximide before addition of 100 nM insulin for 6 h. (B) Hepatocytes were cultured for 16 h in the presence of 5 mM glucose and insulin and then incubated for 15 min in the presence of cycloheximide before addition of 100 μM dibutyryl-cAMP (Bt₂AMPc) for 6 h. The Northern blot presented is representative of two experiments.