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Effect of the dominant negative form of ADD1/SREBP-1c on the binding of ADD1-403 and USF on the E box of the FAS gene in a gel mobility shift assay. Similar amounts of in vitro-translated ADD1/SREBP-1c (lanes 2 to 5) and human USF 1 (lanes 6 to 9) were added to labeled FAS oligonucleotide in the absence (lanes 2 and 6) or presence (lanes 3 to 5 and 7 to 9) of increasing amounts (microliters) of in vitro-translated ADD1-DN.
