FIG. 7.
Effect of a dominant negative form of ADD1/SREBP-1c on T3-induced S14 expression in cultured rat hepatocytes. Hepatocytes were cultured for 16 h in the presence of 5 mM glucose and then incubated for 120 min at 37°C in M199–5 mM glucose either without Ad (control incubation) or with Ad null (30 PFU/cell) or Ad ADD1-DN (30 PFU/cell). The medium was replaced by fresh medium containing 100 nM T3. After 24 h, total RNAs were extracted and analyzed for the expression of S14 and albumin. A representative Northern blot of two culture experiments is shown.