TABLE 1.

Oligonucleotide primers used for amplification and sequencing of 16S rRNA

Primer designation	Sequence (5′ to 3′)	E. coli 16S rRNA positions
8F	TGCAGAGTTTGATCCTGGCTCAG	8–27
133F	CGGGTGAGTAACGCATAGATGACA	110–133
1492F	CCGGGTTACCTTGTTACGACTT	1510–1492
274R	TCTCAGGCCGGATACCCGTCATAGCCT	300–274