FIG. 1.

Transcription of the e(y)1^u1 and e(y)1⁺ genes. (A) Northern blot hybridization of the fragment of e(y)1/TAF_II40 cDNA (Fig. 2A) with mRNA from Oregon R males (lane 1), Oregon R embryos (lane 2), e(y)1^u1/Y males (lane 3), e(y)1^u1/e(y)1^u1 females (lane 4), e(y)1^u1/e(y)1⁺ females (lane 5), embryos from the ♀ e(y)1^u1/e(y)1⁺ × ♂ e(y)1^u1/Y cross (lane 6), C(1)RM,₁yf females (lane 7), and embryos from the ♀ C(1)RM,_yf × ♂ e(y)1^u1/Y cross (lane 8). (B) The same blot hybridized with the Ras2 probe. (C) Relative level of e(y)1/TAF_II40 transcription. The Northern blot was analyzed on a PhosphoImager; signals were normalized according to the results of Ras2 hybridization. The level of transcription in e(y)1^u1/Y males was taken as 1.