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SDS‐PAGE analysis of the multivalent nanobody‐Fc fusion proteins evaluated in this work. Bivalent (Bi), tetravalent (Tetra), and hexavalent (Hexa) nanobodies (produced as Fc fusion proteins) for two nanobodies (VHH‐72 and KC3.ep3) were evaluated via SDS‐PAGE for protein samples either without reduction and heating (−) or with reduction and heating (+).
