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. 2021 Aug 23;12:692831. doi: 10.3389/fmicb.2021.692831

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Copyright © 2021 Liu, Wu, Liang, Lu, Li, Zhang, Li, Zhao, Fu, Hou, Tang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The sensitivity and specificity of SP-NLISA. (A,B) The standard curve and linear range of SP-NLICA for NP detection. (C) The results tested by SP-device and commercial microplate reader exhibited an excellent correlation and no significant difference (R² = 0.999, P < 0.001). (D) The specificity of SP-NLISA for NP detection. The added protein concentration was 1 μg/mL.