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MMQX experiments show evidence for the diffusion of OAA into the PEPCK active site and formation of the PEP product. F o − F o analysis of 40 ms (cyan model) − unmixed (gray model) PEPCK data. F o − F o maps shown at ±3.5 r.m.s.d. (blue, orange). (a) Overview of the asymmetric unit demonstrating that large difference-map peaks are concentrated in the active site. (b) View of the active site, showing difference-map peaks for PEP and relaxation of GDP.
