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Side-chain and GTP-binding pocket motions associated with GDP relaxation 40 ms post-reaction. F o − F o maps are contoured at ±3.5 r.m.s.d. (positive, blue; negative, orange). (a) GTP (gray, umixed) − GDP (cyan, 40 ms) relaxation. (b) Asn292 motion. (c) Thr343 loop motion. (d) Phe517 side-chain breathing. (e) Ala287 motion into phosphate-binding cleft. (f) Arg405 motion away from the active site. (g) Asn403 twist away from the active site.
