TABLE 2.
Comparison of results of microscopy, culture, and the two PCRs for confirmation of the diagnoses of 26 patients with clinically active and histologically confirmed M. ulcerans infection
| Patient code | No. of specimens positive by Ziehl-Neelsen test/total no. of specimens | No. of cultures identified as M. ulcerans/total no. of cultures | No. of specimens positive by 16S rRNA gene PCR/total no. of specimens | No. of specimens positive by IS2404 PCR/total no. of specimens |
|---|---|---|---|---|
| 96-150 | 0/3 | 2/2 | 3/3 | 3/3 |
| 96-162 | 0/2 | 0/2 | 2/2 | 2/2 |
| 96-187 | 1/3 | 2/3 | 3/3 | 3/3 |
| 97-10 | 2/3 | 0/3 | 3/3 | 3/3 |
| 97-72 | 0/4 | 0/4 | 3/4 | 3/4 |
| 97-102 | 0/3 | 1/3 | 2/3 | 3/3 |
| 97-104 | 1/2 | 1/2 | 2/2 | 2/2 |
| 97-105 | 0/2 | 0/2 | 1/2 | 1/2 |
| 97-107 | 0/2 | 0/2 | 2/2 | 2/2 |
| 97-108 | 0/2 | 0/2 | 2/2 | 2/2 |
| 97-117 | 0/1 | 0/1 | 0/1 | 0/1 |
| 97-119 | 1/2 | 0/2 | 1/2 | 1/2 |
| 97-120 | 0/2 | 0/2 | 0/2 | 0/2 |
| 97-121 | 2/2 | 2/2 | 2/2 | 2/2 |
| 97-122 | 0/2 | 0/2 | 2/2 | 2/2 |
| 97-124 | 2/3 | 2/3 | 3/3 | 3/3 |
| 97-125 | 1/3 | 2/3 | 3/3 | 3/3 |
| 97-126 | 0/2 | 0/2 | 1/2 | 1/2 |
| 97-127 | 1/2 | 2/2 | 1/2 | 2/2 |
| 97-129 | 2/2 | 2/2 | 2/2 | 2/2 |
| 97-130 | 5/7 | 5/7 | 7/7 | 7/7 |
| 97-128 | 2/4 | 1/4 | 4/4 | 4/4 |
| 97-187 | 0/3 | 0/3 | 2/3 | 3/3 |
| 97-188 | 0/1 | 0/1 | 1/1 | 1/1 |
| DED | 0/1 | 0/1 | 0/1 | 0/1 |
| GNA | 0/2 | 0/2 | 0/2 | 0/2 |
| Total | 19/65 | 22/65 | 52/65 | 55/65 |