Supplemental Figure S1.

Representative histopathologic features of cholangiocellular lesions developing in Notch intracellular domain (Nicd) and Nicd/NRas mice. The lesions formed in Nicd mice (A–C) are indistinguishable from those developing in Nicd/NRas mice (D–F). Indeed, large cystic lesions (cyst), demarcated by a thin epithelium (B and E), and intrahepatic cholangiocarcinoma (iCCA) can be simultaneously detected in both mouse models. Scale bars: 500 μm (A and D); 100 μm (B, C, E, and F). Original magnification: ×40 (A and D); ×200 (B, C, E, and F). HE, hematoxylin and eosin; w.p.i., weeks post injection.