Supplemental Figure S3.

Chronological and molecular characterization of the lesions developed in Notch intracellular domain (Nicd)/Yes-associated protein (Yap) S127A mice. A: Representative results of the gross and microscopic images of the tumor lesions at different weeks after injection. The immunohistochemistry results indicate the positive immunoreactivity for the proliferation marker Ki-67 and the cholangiocyte marker cytokeratin 19 (CK19). B–D: Quantitative real-time RT-PCR shows up-regulation of the Ck19, Sox9, and Epcam genes in the Nicd/Yap mice of end-stage tumors compared with wild-type (WT) mice. Each gene expression was normalized to the WT mouse liver with 18S rRNA using the −ΔΔCt method. Data are expressed as means ± SD. ∗∗P < 0.01 (t-test). Scale bars = 100 μm. Original magnification, ×200. w.p.i., weeks post injection.