Supplemental Figure S4.

Co-expression of pCMV-Cre (Cre) does not affect Notch intracellular domain (Nicd)/Yes-associated protein (Yap)–driven iCCA formation in mice. Representative liver histologic results of two FVB/N mice 4 and 6 weeks after the hydrodynamic injection of Nicd/Yap/Cre plasmids. Cholangiocellular lesions can already be appreciated in this mouse's liver parenchyma, as indicated by hematoxylin and eosin (HE) staining and positive immunoreactivity for the cytokeratin 19 (CK19) cholangiocellular marker. Scale bars = 50 μm. Original magnification, ×400. w.p.i., weeks post injection; WT, wild type.