Figure 5.

Cholangiocellular lesions developed in Notch1 intracellular domain (Nicd)/Yes-associated protein (Yap) (YapS127A) mice robustly express the injected proto-oncogenes. Representative overview of the lesions formed in a Nicd/YapS127A mouse 8 weeks post injection (w.p.i.) exhibiting positive nuclear and/or cytoplasmic immunoreactivity for pT3EF1α-NICD (MYC-tagged) NICD and YAP proteins. Both the cystic lesions (C) and the intrahepatic cholangiocarcinoma lesions (iCCA) display similar, intense immunolabeling for the two injected proto-oncogenes. The boxed area in the top left panel is shown at higher magnification in the bottom row. Scale bars: 500 μm (top row); 100 μm (bottom row). Original magnification: ×40 (top row); ×200 (bottom row). HE, hematoxylin and eosin.