Figure 8.

Notch1 intracellular domain (Nicd)/Yes-associated protein (Yap) (YapS127A) intrahepatic cholangiocarcinoma (iCCA) tumor formation depends on the mammalian target of rapamycin complex 1 (mTORC1) pathway in mice. A: Experimental study design. B: Survival curve of Raptor^fl/fl mice bearing Nicd/YapS127A tumors. Ablation of Raptor [Nicd/Yap/pCMV-Cre (Cre)] completely prevented tumor formation compared with the control group (Nicd/Yap/pCMV). C and D: Liver weight and liver/body ratio of the pCMV and Cre mouse groups. E: Representative gross image, hematoxylin and eosin (HE), and immunohistochemistry of the pCMV and Cre mice. ∗∗P < 0.01. Scale bars: 100 μm (second column); 50 μm (third, fourth, fifth, and sixth columns). Original magnification: ×200 (second column); ×400 (third, fourth, fifth, and sixth columns).