Table 1.
Antibody List
| Antibody | Source | Catalog no. | Species | Application | Dilution | Retrieval buffer |
|---|---|---|---|---|---|---|
| p-AKTS473 | Cell Signaling Technology (Danvers, MA) | 9271 | Mouse | Western blot | 1:1000 | NA |
| Total AKT | Cell Signaling Technology | 9272 | Mouse | Western blot | 1:1000 | NA |
| mTOR | Cell Signaling Technology | 2983 | Mouse | Western blot | 1:1000 | NA |
| p-4EBP1 | Cell Signaling Technology | 9451 | Mouse | Western blot | 1:1000 | NA |
| 4EBP1 | Cell Signaling Technology | 9644 | Mouse | Western blot | 1:1000 | NA |
| p-RPS6 | Cell Signaling Technology | 4858 | Mouse | Western blot | 1:1000 | NA |
| RPS6 | Cell Signaling Technology | 2217 | Mouse | Western blot | 1:1000 | NA |
| p-ERK1/2 | Cell Signaling Technology | 9101 | Mouse | Western blot | 1:1000 | NA |
| Total ERK1/2 | Cell Signaling Technology | 9102 | Mouse | Western blot | 1:1000 | NA |
| YAP | Cell Signaling Technology | 14074 | Mouse | Western blot | 1:1000 | NA |
| Notch1 | Cell Signaling Technology | 4380 | Mouse | Western blot | 1:1000 | NA |
| MYC tagged | Cell Signaling Technology | 2278 | Mouse | Western blot | 1:1000 | NA |
| RICTOR | Cell Signaling Technology | 2114 | Mouse | Western blot | 1:500 | NA |
| RAPTOR | Cell Signaling Technology | 2280 | Mouse | Western blot | 1:1000 | NA |
| GAPDH | Cell Signaling Technology | 5174 | Mouse | Western blot | 1:3000 | NA |
| CK19 | Abcam (Cambridge, UK) | ab181604 | Mouse | Immunohistochemistry | 1:800 | Sodium citrate |
| HNF4α | Abcam | ab181604 | Mouse | Immunohistochemistry | 1:2000 | Tris-EDTA |
| SOX9 | Abcam | ab185230 | Mouse | Immunohistochemistry | 1:2000 | Sodium citrate |
| Ki-67 | Cell Signaling Technology | 12202 | Mouse | Immunohistochemistry | 1:150 | Sodium citrate |
| p-ERK1/2 | Cell Signaling Technology | 4370 | Mouse | Immunohistochemistry | 1:200 | Sodium citrate |
| p-mTOR Ser2448 | Cell Signaling Technology | 2976 | Mouse | Immunohistochemistry | 1:150 | Sodium citrate |
| MYC-tagged | Cell Signaling Technology | 2278 | Mouse | Immunohistochemistry | 1:100 | Sodium citrate |
| CK19 | Cell Signaling Technology | 12434 | Human | Immunohistochemistry | 1:500 | Sodium citrate |
| RAPTOR | Abcam | ab40768 | Human | Immunohistochemistry | 1:200 | Sodium citrate |
| p-4EBP1 | Cell Signaling Technology | 2855 | Human | Immunohistochemistry | 1:300 | Sodium citrate |
| Notch1 | Lifespan Biosciences (Seattle, WA) | LS-C114369 | Human | Immunohistochemistry | 1:100 | Sodium citrate |
| YAP | Cell Signaling Technology | 14074 | Human | Immunohistochemistry | 1:200 | Sodium citrate |
| p-YAP | Cell Signaling Technology | 13008 | Human | Immunohistochemistry | 1:300 | Sodium citrate |
| CTGF | Atlas Antibodies (Stockholm, Sweden) | AMAb91366 | Human | Immunohistochemistry | 1:100 | Sodium citrate |
CK, cytokeratin; CTGF, connective tissue growth factor; ERK, extracellular signal-regulated kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HNF4α, hepatocyte nuclear factor 4α; mTOR, mammalian target of rapamycin; NA, not applicable; p-, phosphorylated; MYC tagged, pT3EF1α-NICD; YAP, Yes-associated protein.