KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Rapamycin (Sirolimus)	LC Laboratories	Cat#R-5000
HaloTag ligand - Janelia Fluor 552 (JF552)	Zheng et al., 2019	N/A
HaloTag ligand - Janelia Fluor 646 (JF646)	Grimm et al., 2015	N/A
Deposited data
Raw localization/tracking data (DiaTrack output) and main processing scripts	This paper	Mendeley Data DOI: 10.17632/3xktk72wbd.1
Experimental models: Organisms/strains
S. cerevisiae YAR295 (Halo-H2B)	Ranjan et al., 2020	N/A
S. cerevisiae YAR302 (Free Halo)	Ranjan et al., 2020	N/A
S. cerevisiae YAR270 (rpb1-AA)	Ranjan et al., 2020	N/A
See Table S3 for S. cerevisiae strains generated for this study.	This paper	N/A
Oligonucleotides
See Table S4 for oligonucleotides used for tagging S. cerevisiae PIC components for SMT imaging and Anchor Away.	This paper (from IDT)	N/A
Recombinant DNA
pFA6a-TRP1-pGAL1-HBH	Booher and Kaiser, 2008	RRID:Addgene_26886
N-terminal HaloTag: pFA6a-TRP1-pGAL1-HALO	This paper	N/A
C-terminal HaloTag: pBS-SK-Halo-NatMX	This paper	N/A
N-terminal FRB-GFP tag for AA: pFA6a-TRP1-pGAL1-FRB-GFP	This paper	N/A
C-terminal FRB-GFP tag for AA: pFA6a-FRB-GFP-kanMX	This paper	N/A
Software and algorithms
Sojourner	Wu Lab	https://github.com/sheng-liu/sojourner
Fiji	Schindelin et al., 2012	http://fiji.sc
Prism version 8 for Mac	GraphPad Software	www.graphpad.com
DiaTrack version 3.05	Vallotton and Olivier, 2013	N/A