Figure 7.

miR-17-5p sensitized xenograft tumor to irradiation and inhibited irradiation-induced autophagy. (A) Nude mice were subcutaneously injected with U87 cells infected with control lentivirus vector or miR-129-5p overexpression lentivirus vector. The tumors were irradiated (10 Gy) on day 15 when the average tumor volume reached about 500 mm3. Tumor volume was monitored over time as indicated. (B) The tumors were excised on day 40. miR-129-5p overexpression caused a significant decrease in tumor volume. (C–F) the autophagy activity was determined by Western blot for beclin-1, Atg7, and p62. Relative density of protein bands was measured using ImageJ and normalized to GAPDH. Data are presented as mean ± SD from at least three independent experiments. **p < 0.01 compared with LV-scramble group.