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. 2021 Sep 7;28(7-8):715–729. doi: 10.3727/096504020X16037124605559

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Copyright © 2021 Cognizant, LLC.

This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.

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The C8orf34-as1–miR-671-5p–MFAP4 axis is critical for LUAD cell invasion and stemness. (A–C) Pearson correlation analysis of the relationship between MFAP4, miR-671-5p, or C8orf34-as1 expression and the mRNAsi score. (D) Transwell assays were performed to determine invasion in A549 and H1299 cells after different treatments. ***p < 0.001. (E) Sphere formation assays were performed to analyze stemness in A549 and H1299 cells after different treatments. ***p < 0.001. (F) Western blot assays were performed to analyze the effect of C8orf34-as1/miR-671-5p/MFAP4 on three key stemness-related genes (SOX2, OCT4, and NANOG). (G) Real-time quantitative polymerase chain reaction (RT-qPCR) and immunoblot showing decreased MFAP4 expression in spherical cells compared to adherent cells, **p < 0.01.