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. 2021 Sep 7;28(7-8):763–778. doi: 10.3727/096504021X16135618512563

Figure 4.

Figure 4

LMP1 enhances the ability of wt-p53 to induce expression of EBV lytic proteins through ERKs, p38, and JNKs in AGSEBV cells. (A) AGS-EBV cells were transfected with a plasmid containing pSG5-LMP1 or a control pSG5 vector for 48 h. Western blotting analysis was performed to detect the levels of LMP1, total MAPKs and p53, and phosphorylated MAPKs and p53. (B–D) Western blotting analysis for EBV lytic proteins and phosphorylated p53 in AGS-EBV cells pretreated with PD98059, SB202190, or Sp600125 for 24 h at the concentrations indicated. (E) AGS-EBV cells were transfected with plasmid expressing LMP1 or empty vector for 48 h. Coimmunoprecipitation (Co-IP) assay using anti-p53 antibody was performed to analyze the interactions of p53 with Sp1 and SMAD2/3. The resulting immunocomplexes were recovered and analyzed by Western blotting analysis. β-Actin was used as an internal control.