Figure 2.

The effects of S100A2 knockdown on the proliferation, invasion, and EMT in BC cells. MDA-MB-453 and MDA-MB-468 cells were transfected with si-S100A2 or si-NC for 48 h. (A) The protein expression of S100A2 was determined via Western blot. (B) Cell proliferation was assessed by a BrdU assay. (C) The protein expressions of CDK2, cyclin E1, and p21 were determined by Western blot. (D) The invasion of BC cells was assessed by Transwell assay. (E) The mRNA levels of MMP-2, MMP-9, and TIMP-1 were detected by quantitative RT-PCR assay. (F) The expressions of E-cadherin, vimentin, and N-cadherin were detected by Western blot. All data are presented as means ± SEM, n = 6. #p < 0.05, ##p < 0.01 versus si-NC.