Effects of miR-325-3p knockdown on the invasion and EMT of BC cells. MDA-MB-231 and MCF7 cells were transfected with miR-325-3p inhibitor or anti-miR-NC for 48 h. (A) The invasion of BC cells was assessed by Transwell assay. (B) The mRNA levels of MMP-2, MMP-9, and TIMP-1 were detected by PCR assays. (C) The expressions of E-cadherin, vimentin, and N-cadherin were detected by Western blot assays. All data are presented as mean ± SEM, n = 6. #p < 0.05, ##p < 0.01, ###p < 0.001 versus anti-miR-NC.