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. 2021 Sep 7;28(7-8):731–744. doi: 10.3727/096504020X16100888208039

Figure 8.

Figure 8

Overexpression of S100A2 partially promoted cell proliferation, invasion, and EMT in miR-325-3p-overexpressing BC cells. BC cells were transfected with either miR-325-3p mimic with or without pcDNA-S100A2 vector. (A) The protein expression of S100A2 was determined by Western blot. (B) Cell proliferation was assessed by CCK-8 assay. (C) The mRNA and protein expressions of PCNA, CDK2, cyclin E1, and p21 were determined by qRT-PCR and Western blot, respectively. (D) The invasion of BC cells was assessed by Transwell assay. (E) Total secretions of MMP-2, MMP-9, and TIMP-1 in the culture supernatants were detected by ELISA assays. (F) The expressions of E-cadherin, vimentin, and N-cadherin were detected by Western blot assays. All data are presented as mean ± SEM, n = 6. *p < 0.05, **p < 0.01, versus miR-NC; #p < 0.05, ##p < 0.01 versus miR-325-3p mimic + pcDNA3.1.