Figure 8.

CDX and CAG sites sensitise WREs to Wnt signalling. (A) Cartoons of the five synthetic WREs with different combinations of TCF, CDX and CAG sites. Constructs shown were cloned upstream of a minimal promoter to generate luciferase reporters. (B–D) Relative activity levels of the synthetic WREs in HEK293T, LS174T and HeLa cells. HEK293T (B) and HeLa cells (C) were transfected with indicated reporters and an empty vector or β-catenin* expression plasmid. Fold activation by β-catenin* was compared. LS174T cells were transfected with indicated reporters, and activity was normalised to that of the empty vector with only the minimal promoter (D). Each bar represents mean ± SD from three replicates (N = 3) P< 0.05; **P< 0.005; ***P< 0.001; ****P < 0.0001; n.s. P > 0.05.