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. 2021 Apr 28;49(15):8407–8418. doi: 10.1093/nar/gkab298

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Circularization of the Ori macrodomain by Flp-POP cloning. (A) Scheme for the pop-out of Chr^LR+Ter by Flp-POP cloning and the concurrent generation of Chr^Ori. The resultant bipartite-genome strains gained resistance to chloramphenicol via the fused resistance gene. Two types of BAC vectors, pVtu9xT and pPKOZ-attB, were used to develop bipartite-genome strains RGF123 and RGF124, respectively. (B) PFGE analysis of the chromosomes of two colonies of RGF123 [Chr^Ori & Chr^LR+Ter (pVtu9xT)] and two colonies of RGF124 [Chr^Ori & Chr^LR+Ter (pPKOZ-attB)]. The DNA size markers used for PFGE are Hansenula wingei chromosomes.