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. 2021 Apr 28;49(15):8407–8418. doi: 10.1093/nar/gkab298

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Partial genome swapping via conjugation. The donor strain carries a helper plasmid for conjugation, pBAD-traRP4min, indicated with a red circle. The Chr^Ori and Chr^Ter chromosomes contain an oriT sequence whose direction of transfer is indicated with red arrows. (A) The Chr^Ori of RGF159 was swapped by the Chr^Ori of RGF156 (RGF152 with the helper plasmid). Note that the zeo marker is the last gene transferred via conjugation from the oriT sequence. (B) The Chr^Ter of RGF152 was swapped by the Chr^Ter of RGF161 (RGF159 with the helper plasmid). The resultant strains RGF160 (A) and RGF162 (B) shared the same genotype. Selection marker gene loci were confirmed by colony PCR analyses as shown in the bottom panels (A, B).