Table 1.
Plasmids, BAC vectors and chromosomes used in this study
| name | Description |
|---|---|
| pMW118-Aba | Helper plasmid for λ-red recombination with recA and λ bet-exo cloned under araC-ParaBAD |
| pMW118spec-flp-int* | Helper plasmid for Flp-POP cloning with flippase and HK022 int cloned under araC-ParaBAD |
| pMW118gent-flp-int | pMW118spec-flp-int derivative with a gent gene |
| pBAD-traRP4min* | Helper plasmid for RP4 tra expression, ΔoriT |
| pPKOZ-attB* | BAC vector composed of oriC, sopABC, kan, attB |
| pPKOZ-attB-spec | BAC vector derived from pPKOZ-attB with a spec gene |
| pVtu9xT* | BAC vector composed of V. tubiashii ori2-par2, kan, attB, oriT |
| pVtu9xT-PEM7-spec | BAC vector derived from pVtu9xT with a replacement of kan by spec |
| pVtu9xF | BAC vector composed of V. tubiashii ori2-par2, kan, attB, a remnant FRT |
| ChrLR (RGF152/YST01) | ChrLR (pPKOZ-attB-spec) purified from RGF152 or YST01 The cloned regions: from leuD to ybgL through rrnH and from yqeF to menF through rrnG. The selection markers: kanamycin, spectinomycin, and tetracycline |
| ChrTer (HF053) | ChrTer (pVtu9xF) purified from HF053 The cloned region: from gltA to glpC through dif The selection marker: kanamycin |
| ChrOri (HF054) | ChrOri purified from HF054 The cloned region: from lysS to polB through oriC with endA::tet The selection markers: tetracycline, hygromycin, chloramphenicol, and Zeocin |
*These plasmids were reported previously (32).