Figure 4.
The C-terminal domain of the G. lamblia H2A contributes to the nucleosomal DNA end flexibility. (A) The G. lamblia NCP, the H2AG.lambliaCTD NCP, and the human canonical NCP were reconstituted with the 601L DNA and treated with MNase for the indicated times. The DNA fragments were deproteinized and analyzed by non-denaturing polyacrylamide gel electrophoresis with ethidium bromide staining. The experiments were independently repeated three times, and the reproduced results are shown in Supplementary Figure S6. (B) Quantitation of the MNase assay. Undigested DNA was quantitated from stained gel images and plotted as mean and standard deviation (n = 3).