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. 2021 Aug 5;49(15):8934–8946. doi: 10.1093/nar/gkab644

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — LANA acidic patch binding peptide does not bind to the G. lamblia NCP. (A) GST-LANA (1, 2, 4, 8, 16 and 32 μM) or GST (32 μM) were incubated with the human NCP (0.5 μM) or the G. lamblia NCP (0.5 μM). The complex formation was analyzed by non-denaturing PAGE with ethidium bromide staining. (B) GST-LANA (1, 2, 4, 8, 16 and 32 μM) was incubated with the human NCP (0.5 μM) or the acidic patch-defective NCP (0.5 μM) containing a 145 bp Widom 601 DNA, as in A. An independent replicate experiment is shown in Supplementary Figure S9.