Skip to main content
. 2021 Aug 5;49(15):8743–8756. doi: 10.1093/nar/gkab652

Figure 6.

Figure 6.

The eIF3-binding RPE i. of uORF1 genetically interacts with eIF4G in vivo. (A) Schematics showing uORF1-only RaP-NiP construct and its derivatives with substitutions in RPE i (uORF1-SUB40; substitution of nt -40 through -49 of RPE i. with the complementary sequence) or in RPE ii. (uORF1-CAAII; substitution of whole RPE ii. sequence [nt –55 through –76] with seven CAA repeats). (B) The YMP53, YMP55, YMP56 and YMP91 strains described in Figure 3 were introduced with the uORF1-only RaP-NiP construct or its derivatives shown in panel A and treated as described in Figure 1B. Relative qPCR product levels (in %) of the uORF1 segment recovered from each strain were processed as described in Figure 2B with the values of the wt uORF1-only strain expressing His-eIF4G1 set to 100% (*P< 0.05, **P< 0.005, ***P< 0.0005). (C) The strain YMP63 described in Figure 1C was introduced with the uORF1-only RaP-NiP construct or its derivatives shown in panel A and treated as described in panel B (*P< 0.05, **P< 0.005).