Figure 4.

Effect of chromatin status on the LacI-luciferase reporter assay. (A) Conceptual design of Chromatin assessability. The computed peak with the four scoring terms based on peak calling results and peak quantitative score. Each scoring term provides information on a total peak score and number of probes. High level, score ≥5; medium level, score = 3–4; low level, score = 2; silent, score = 0. Within five randomly selected genes, the six single guide RNAs were designed for each of the four groups. Correlations between cleavage efficiencies according to the LacI-luciferase reporter and indel frequencies measured using deep sequencing were determined for a total of 118 sgRNAs targeting endogenous sites covering all four groups (high, medium, low, and silent) (B), high group only (C), medium group only (D), low group only (E), and silent group only (F).