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. 2021 Jul 30;49(15):8556–8572. doi: 10.1093/nar/gkab626

Figure 3.

Figure 3.

MiR-339 reactivates TSG GPER1 and inhibits proliferation of 4175 and T47D breast cancer cells. (A, B) MiR-339 reactivated its targeted TSG GPER1 expression (A) but downregulated GPER1 by transfecting miR-339 inhibitors (B) in 4175 cells through RT-PCR detection. (C) MiR-339 and its inhibitors respectively reactivated and downregulated the GPER1 protein expression levels in 4175 cells by western blot assay. (D–F) the expression of targeted TSG GPER1, was reactivated by miR-339 (D) and downregulated by miR-339 inhibitors in T47D cells (E) through RT-PCR detection and Western blot assay (F). (G, H) MiR-339 repressed the proliferation cells (G) and colony formation ability (H) of 4175 cells. (I) MiR-339 did not impact on breast cancer cell migration ability. (J, K) GPER1 knock-down by shGPER1 increased the proliferation (J) and colony formation ability (K) in 4175 cells. (L) GPER1 showed no impact on breast cancer cell migration ability. Data are presented as Mean ± SD of experiments conducted in triplicate. ****P < 0.0001, **P < 0.01, *P < 0.05. “ns” is representative of no significance.