Table 2.

Diffusion coefficients and amplitudes for 1-D diffusion modes of Fis proteins along DNA in vitro¹

Proteins	[Kglu] (mM)	D1 (μm2 s–1)	D2 (μm2 s–1)	A1: A2 (%)
Fis WT	50	0.0092 ± 0.0004
Fis R85A	50	0.040 ± 0.009*	0.27 ± 0.02	32 ± 6: 68 ± 8
Fis K90A	50	0.0134 ± 0.0004*
Fis WT	150	0.0138 ± 0.0004	0.156 ± 0.007	76 ± 2: 24 ± 1
Fis R71A	150	0.0259 ± 0.0006*	0.24 ± 0.02*	88 ± 2*: 12.0 ± 0.9*
Fis N84A	150	0.0132 ± 0.0003	0.122 ± 0.007*	85 ± 2*: 15.3 ± 0.9*
Fis K90A	150	0.0098 ± 0.0002*	0.242 ± 0.006*	68.8 ± 0.9*: 31.2 ± 0.6*

¹The parameters were obtained by fitting the displacement distribution of single molecules along DNA in vitro by Equation (1). Bar graphs of the data are provided in Supplementary Figure S10B.

*Significant difference between mutants and WT was determined using Welch’s t test with α = 0.05; no asterisk indicates no significant difference. We note that statistically significant variations in the values of the mutants versus wild-type proteins obtained by the analyses of the imaging data may not necessarily reflect biologically meaningful differences.