Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 6;11:17705. doi: 10.1038/s41598-021-97162-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Mortalin expression controls axonal fate against a distal oxidative stress insult. Rat embryonic primary cortical neurons were grown in microfluidic devices for 12 DIV. Neurons were transduced on DIV 3 with lentiviral vectors (LV) to induce over- (LV-Mortalin) or under- (LV-shMortalin, clone A) expression of Mortalin. A lentiviral vector expressing a shRNA with scramble nucleotide sequence was used as a control (LV-shScr). On DIV 11, neurons were treated or not with 1 μM rotenone applied in the axonal chamber (+ rotenone) for 24 h and fixed on DIV 12. (A) Pictures of βIII-tubulin staining were randomly taken within axonal chambers (4 by condition) and the axonal fragmentation indexes were measured for each picture as the number of axonal dots per unit of tubulin staining. (B) Results are presented as means ± SD of 4 to 10 independent microfluidic devices, from 4 independent neuronal cultures. *p < 0.05, **p < 0.01, ****p < 10⁻⁴, by 1-way ANOVA with Sidak’s multiple comparison post-hoc test.