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. 2021 Sep 6;11:17705. doi: 10.1038/s41598-021-97162-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mortalin modulates mitochondrial morphology in neuronal extensions. Rat embryonic primary cortical neurons were transduced on DIV 3 with lentiviral vectors (LV) to induce over- (LV-Mortalin) or under- (-LV-shMortalin, clone A) expression of Mortalin. A lentiviral vector expressing a shRNA with a scramble sequence was used as a control (LV-shScr). (A) Neurons were fixed on DIV 12 and both neuronal and mitochondrial networks were revealed by staining with, respectively, Tom20 and βIII-tubulin (βIII-Tub). Pictures were randomly taken and mitochondrial lengths were measured in all neuronal extensions. (B) For each neuron, the sum of the lengths of all measured mitochondria was calculated (mitochondrial network), within which the relative proportions of short (< 2 m), medium (2–6 m) and long (> 6 m) mitochondria were considered. The graph represents means ± SD of 18 to 40 neuronal mitochondrial networks, from 4 independent neuronal preparations. *p < 0.05, **p < 0.01, ***p < 0.001, by 2-way ANOVA, Kruskal Wallis test and Dunn’s multiple comparisons as post-hoc tests.