Fig. 3. Orientation changes of the NRLC and CRLC probe in response to shortening of Ca²⁺-activated muscle.

a Upper panel: muscle length change (ΔL₀); middle panel: normalized force; bottom panel: <P₂> from the NRLC (red) and CRLC probe (orange). b Length-dependence of the change in <P₂> (Δ<P₂>) from NRLC measured at the end of the <P₂> transient. Data are shown as means ± s.e.m. (n = 5 for −20% ΔL₀ and −10% ΔL₀; and n = 4 for −5% ΔL₀ and −15% ΔL₀). c Plot of steady state <P₂> change (Δ<P₂>) of NRLC versus normalized isometric force (n = 23; pooled data from n = 5 trabeculae). Solid line represents linear regression with 95% confidence interval indicated by dotted lines. Pearson correlation coefficient (r) and p value for a two-tailed student’s t test are shown on the bottom right. Source data are provided as a Source Data file.