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. 2021 Sep 6;12:5263. doi: 10.1038/s41467-021-25634-z

Fig. 3. The β-Catenin destruction complex regulates CRBN-mediated CK1α degradation.

Fig. 3

a Extracts of HEK cells treated with Wnt3a for various lengths of time were evaluated by immunoblotting. Quantitation of immunoblots (mean ± SEM, n = 3 independent experiments) is shown. Levels of phosphorylated β-Catenin were normalized to that of total β-Catenin. b HEK cells were transfected with control (CTRL) siRNA or one of three distinct CRBN siRNA, followed by PBS or Wnt3a treatment for 24 h. Extracts of these cells were evaluated by immunoblotting. c CRBN was immunoprecipitated from extracts of HEK cells treated with PBS or Wnt3a in the presence of MG132 for 4 h, followed by immunoblotting of the indicated proteins. d HEK cells were co-transfected with control (CTRL) siRNA or smart-pool AXIN1 or APC siRNA, along with one of two distinct CRBN siRNA. Extracts of these cells were evaluated by immunoblotting. A representative immunoblot (n = 3 independent experiments) is shown in b–d.