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. 2021 Sep 7;7:81. doi: 10.1038/s41421-021-00316-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–f Immunofluorescence staining of human EPS-blastoids for EPI lineage marker (OCT4), TE lineage markers (CK8 and GATA2/3), and PE lineage marker (GATA6), Scale bar = 50 μm. g Model of immunofluorescence staining of human blastocyst for OCT4 in EPI, GATA6 in PE, GATA2/3, and CK8 in TE lineages, Scale bar = 50 μm. h–j Quantification of the percentage of human blastoids with correct allocation of OCT4 and CK8 (h), GATA6 (i), and all three markers (j). Data in i, data are means ± SD (n = 172 blastoids). *P < 0.05. Data in i, j, data are means ± SD (n = 131 blastoids).