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. 2021 Sep 6;6:113. doi: 10.1038/s41541-021-00376-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — B cells from healthy donor PBMCs were stained with eOD-GT8-60mer probes directly conjugated with fluorophores and sorted for IgG^neg/eOD-GT8-60mer⁺⁺ eOD-GT8^KO-60mer^neg B cells, followed by BCR sequencing. a–d Flow cytometry of sorted cells in donor 4 (a), donor 5 (b), donor 6 (c), and donor 7 (d). e–h IGHV gene usage distribution of paired IgG^neg/eOD-GT8-60mer⁺⁺ eOD-GT8^KO-60mer^neg B cells from donor 4 (e), donor 5 (f), donor 6 (g), and donor 7 (h). Bars corresponding to IGHV1-2 genes are colored in red. i Isotype distribution of LCs coexpressed with IGHV1-2. j LCDR3 lengths of IGKV LCs coexpressed with IGHV1-2. k As in h, but for IGLV LCs. l LCDR3 sequences of naive 5-AA IGKV LCs. m IGKV and IGLV usage distribution among eOD-GT8-60mer⁺⁺ eOD-GT8^KO-60mer^neg, and 5-AA LCDR3 VRC01-class naive B cells. Text color coding is as in Fig. 1k. IGKV3-11 indicated by an asterisk has not been directly observed in VRC01-class bnAbs, although the LC of VRC01 was originally annotated as IGKV3-11³⁷ due to its high similarity to IGKV3-20. n IOMA-class B cells sorted with eOD-GT8-60mer probes.