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. 2021 Sep 6;4:1038. doi: 10.1038/s42003-021-02528-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Representative western blot of Piezo1-GFP and S217L expressed in HEK293 and Neuro2a cells (N2a). b Representative western blot of Piezo1-GFP, Piezo1mCherry, S217L-GFP, S217L-mCherry, and co-expression of Piezo1-GFP/Piezo1-mCherry, Piezo1-GFP/S217L- mCherry, Piezo1-mCherry/ S217L-GFP. The GFP and mCherry signal are shown separately. c Quantification of peak current elicited per patch of all combinations expressed in Piezo1^-/- HEK293T cells. Asterisk (*) denotes statistical significance p < 0.05 determined by Kruskal–Wallis test with Dunn’s post-hoc test.