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. 2021 Sep 6;4:1038. doi: 10.1038/s42003-021-02528-w

Table 1.

Primers used for site directed mutagenesis in this study.

Primer Sequence
hP1-N2294Q Sense: gcgggagctctaccagggcacggccgaca
hP1-N2294Q Anti-sense: tgtcggccgtgccctggtagagctcccgc
hP1-N2331Q Sense: ggccctggcccctcagagcactgcacgg
hP1-N2331Q Anti-sense: ccgtgcagtgctctgaggggccagggcc
hP1-L939M Sense: tggcctcgaataccatcagcagcagcacttg
hP1-L939M Anti-sense: caagtgctgctgctgatggtattcgaggcca
hP1-G2029R Sense: aaggccagcttgcgcagcacggtcttg
hP1-G2029R Anti-sense: caagaccgtgctgcgcaagctggcctt
hP1-S217L Sense: catcgcccaccccttggccctctc
hP1-S217L Anti-sense: gagagggccaaggggtgggcgatg
hP1-Y2022A Sense: tgaccgcgccctcgccctgcgcaagacc
hP1-Y2022A Anti-sense: ggtcttgcgcagggcgagggcgcggtca
hP1-K2502R Sense: ggtagaggaagatgagcctggcgtacaactcct
hP1-K2502R Anti-sense: ggaggagttgtacgccaggctcatcttcctctac
hP1_N295Q_Sense cgtgggggctggagcactgggtgggaccc
hP1_N295Q_Anti-sense cttcgtgggtcccacccagtgctccagcc
hP1_N658Q_Sense gtgaagccagtgagctggcgccagtaggcag
hP1_N658Q_Anti-sense ctgcctactggcgccagctcactggcttcac
hP1_N885Q_Sense gaagggctcggtgcactggctggaatactcctg
hP1_N885Q_Anti-sense caggagtattccagccagtgcaccgagcccttc
hP1_N892Q_Sense gcaagttggtgctctgggggaagggctcgg
hP1_N892Q_Anti-sense gcccttcccccagagcaccaacttgctgcc
hP1_N1095Q_Sense gaggttggtggactggggggcccggaag
hP1_N1095Q_Anti-sense cttccgggccccccagtccaccaacctc
hP1_N1222Q_Sense ggagatgatgacggtgacctggtacagaatgaggcagtc
hP1_N1222Q_Anti-Sense gactgcctcattctgtaccaggtcaccgtcatcatctcc
mP1_Ct_GFP fusion-Sense tggacacgtgagagggagacaaccatggtgagcaag
mP1_Ct_GFP fusion-Anti-sense cttgctcaccatggttgtctccctctcacgtgtcca