Fig. 1. Monocytes contribute to BAT macrophage pool at a steady state.

A Single-cell RNA-Seq analysis of BAT CD45⁺ cells from 7−8-week-old male mice. B UMAP representations of genes used to identify cell types among BAT CD45⁺ cells. C Heatmap showing normalized expression levels of marker genes helping to identify cell types present in the data. D Flow cytometry plot showing CCR2^GFP expression among BAT Ly6C^+/– MHCII^+/– cells (gated on CD45⁺ CD11b⁺ CD64⁺ cells). E Flow cytometry plot showing TdTomato expression among BAT Ly6C^+/– MHCII^+/– cells (gated on CD45⁺ CD11b⁺ CD64⁺ cells) from CCR2^creERT2/+ and CCR2^creERT2/GFP mice 48 h after tamoxifen gavage. F Quantification of TdTomato⁺ cells among CD45⁺ CD64⁺ CD11b⁺ cells (p = 0.0002) and macrophages (CD45⁺ CD64⁺ CD11b⁺ Ly6C^–) (p < 0.0001) in the BAT of CCR2^creERT2/+ (CCR2^+/–, n = 12) and CCR2^creERT2/GFP (CCR2^–/–, n = 7) mice 48 h after tamoxifen gavage. G Quantification of macrophages in the BAT of CCR2^+/– (n = 18) and CCR2^–/– (n = 9) mice, p = 0.8997. Panels D, E, F, and G represent pooled data from two independent experiments. All data are represented in means ± SEM. Two-tailed Mann−Whitney tests were used to determine statistical significance. ns p > 0.05; *p < 0.05; **p < 0.01. Source data are provided as a Source Data file.