Skip to main content
. 2021 Sep 6;12:5255. doi: 10.1038/s41467-021-25616-1

Fig. 4. Monocyte depletion prevents the expansion of lipolysis-deficient BAT.

Fig. 4

A Schematic representation of the experimental procedure used for MC-21-mediated monocyte depletion. B Representative dot plots (left) and quantification (right) of BAT monocytes in MC-21 (n = 12) or vehicle-treated (n = 16) Adipo∆/∆ mice. p < 0.0001. C Frequency and numbers of BAT macrophages in MC-21 (n = 13) or vehicle-treated (n = 18) Adipo∆/∆ mice. p = 0.0001 (left) and p = 0.3189 (right). D Brown adipose tissue weight in MC-21 (n = 5) or vehicle-treated (n = 6) control mice, and MC-21 (n = 14) or vehicle-treated (n = 18) Adipo∆/∆ mice. p < 0.0001 (vehicle-treated control and Adipo∆/∆ mice) and p = 0,0002 (vehicle-treated and MC-21-treated Adipo∆/∆ mice). E Representative images of H&E-stained BAT and quantification of multilocular areas in MC-21 (n = 14) or vehicle-treated (n = 17) Adipo∆/∆ mice. Scale bar = 100 μm. p = 0.0053. Data were derived from three pooled independent experiments. All data are represented in means ± SEM. Two-tailed Mann−Whitney tests were used to determine statistical significance. ns p > 0.05; *p < 0.05; **p < 0,01; ***p < 0.001. Source data are provided as a Source Data file.