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. 2021 Sep 6;12:5296. doi: 10.1038/s41467-021-25673-6

Fig. 2. The ocn promoter-driven GFP expression in ECs.

Fig. 2

a–c The ocn promoter-driven GFP expression was detected in aorta and other tissues of hOC-GFPtpz mice, determine by Western blotting (a), cross-section staining with aorta and skeletal muscle (b) and en face staining with aorta (c). SKM, skeletal muscle. Hrt, heart. Arrows indicate GFP-positive ECs. L, lumen. M, media. V, vessel. Negative control images with tissues of non-transgenic mice are shown in Supplementary Figure 2j. d GFP levels in bone and aorta isolated from hOC-GFPtpz mice at indicated age. e GFP levels in bone, aorta and skeletal muscle isolated from streptozotocin (STZ)-induced diabetic or control hOC-GFPtpz mice. f GFP levels in bone, aorta and skeletal muscle isolated from LRP1 eKO; hOC-GFPtpz or WT; hOC-GFPtpz mice. Scale bar, 20 μm. n = 3 (d–f). NS, not significant. Data are presented as mean ± SEM. Analysis was two-way ANOVA followed by Fisher’s LSD multiple comparison test (d–f).