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. 2021 Sep 6;12:5281. doi: 10.1038/s41467-021-25565-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The maximum likelihood tree was generated based on the concatenated 50% top-ranked orthologs (n = 38, 10,896 sites) with IQ-Tree (v2.0.7) using LG + F + R + C60 model. The tree includes 152 Woesearchaeota genomes and is rooted by 30 Pacearchaeota genomes. 49 MAGs generated in this study are marked by the star. Black dots denote nodes with UFBOOT support values ≥95% and SH-aLRT values ≥80%. Scale bar shows the average number of substitutions per site. The minimum number of orthologs in Woesearchaeota MAGs is 21. J072 and Yap5000.bin1.9 were ungrouped as they did not form well-supported clade (UFBOOT < 95% and SH-aLRT <80%). Source data are provided as a Source Data file.