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. 2021 Sep 6;12:5308. doi: 10.1038/s41467-021-25675-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Colour indicates soil treatment (control = grey, 1 year = light blue, 10 years = blue). On top, graph NMDS representing archaea/bacteria, fungi and AMF (arbuscular mycorrhizal fungi). All the NMDS plots show the similar patterns, with an effect of drought (respectively, p = 0.014, p = 0.01, p = 0.015; PERMANOVA results, n = 4) and with a clear separation of the 10-year treatment from 1 year and control. The bottom graphs show results from the differential abundance analysis for control vs 1 year (top row) and control vs 10 years (bottom row). Circles in red represent taxa that had a significant differential abundance compared to the control, while in grey taxa that were not significant. Similarly to the NMDS plots, control vs 1 year results show little difference (only in the fungal community of Basidiomycota), while control vs 10 years shows strong differences at all levels (most affected groups being: Acidobacteria, Actinobacteria, Proteobacteria, Ascomycota, Basidiomycota, Diversisporales and Glomerales).