Figure 3.

Piezo1 channel activator induces [Ca²⁺]_i increases in human odontoblasts. (A,C) Representative traces of transient [Ca²⁺]_i increase in response to 2μM Yoda1 in standard extracellular solution are shown. Addition of 1μM Gd³⁺ (A) or 10μM Dooku1 (C) inhibited Yoda1-induced [Ca²⁺]_i increases. In each figure, the boxes indicate the timings when the test solution, including pharmacological activator (black boxes at the top) or inhibitors (white boxes at the top), were applied. (B,D) Bar graphs representing values of F/F₀ for the increases in [Ca²⁺]_i by 2μM Yoda1 with (gray columns) or without (open columns) 1μM Gd³⁺ (B) or 10μM Dooku1 (D). The pharmacological activators and inhibitors used are indicated on the left side of the graphs. The resting value is defined as F/F₀=1.0. Each bar indicates the mean±SE of six (B) and 12 (D) experiments. Asterisks denote statistically significant differences between columns (shown by solid lines): ^*p<0.05.