Figure 6.

Piezo1 channel activation decreases the mineralization levels. (A) Human odontoblasts were cultured for 28days in mineralization medium with 2μM Yoda1 (second left), 2μM Yoda1 and 10μM Dooku1 (third left), 10μM Dooku1 (right most), or without these channel modifiers (left most) at pH 7.4, and stained by Alizarin red (red, calcium deposition). (B) Mineralization levels without (control; upper most column) or with 2μM Yoda1 (second column), 2μM Yoda1 and 10μM Dooku1 (third column), and 10μM Dooku1 (lower column), assessed by Alizarin red staining. The mineralization levels were 4.80±0.13 I/I₀ in the absence of Piezo1 channel modifiers (as controls), while those 1.96±0.04 I/I₀ with 2μM Yoda1, 2.63±0.12 I/I₀ with 2μM Yoda1 and 10μM Dooku1, and 3.11±0.18 I/I₀ with 10μM Dooku1. Each column denotes the mean±SE from eight experiments. Statistically significant differences between columns (shown by solid lines) are indicated by asterisks. ^*p<0.05.