Table 1.
Target genes and their primers used in this study.
| Primers | Sequence (5′-3′) | Products sizes (bp) | Annealing (ºC) | References |
|---|---|---|---|---|
| bla IMP | Fw- GGAATAGAGTGGCTTAAYTCTC | 232 | 52 | (17) |
| Rv- GGTTTAAYAAAACAACCACC | ||||
| bla VIM | Fw- GATGGTGTTTGGTCGCATA | 390 | 59 | (18) |
| Rv- CGAATGCGCAGCACCAG | ||||
| bla NDM | Fw- GGTTTGGCGATCTGGTTTTC | 621 | 52 | (17) |
| Rv- CGGAATGGCTCATCACGATC | ||||
| 16S rRNA (P. aeruginosa) | Fw- GGGGGATCTTCGGACCTCA | 956 | 58 | (19) |
| Rv- TCCTTAGAGTGCCCACCCG | ||||
| 16S rRNA (A. baumannii) | Fw- TTTAAGCGAGGAGGAGG | 240 | 56 | (20) |
| Rv- ATTCTACCATCCTCTCCC | ||||
| 16S rRNA (S. maltophilia) | Fw- AGTTCGCATCGTTTAGGG | 762 | 58 | (21) |
| Rv- ACGGCAGCACAGAAGAGC |